In brief, flow cytometry is a technique for rapid quantitative analysis and sorting of multi-parameters for cells or biological particles at a quick straight flow.
From the start of envisioning the arrival of the first tool, technological and scientific workers have made unremitting efforts. With the rapid development of related technologies, ideal flow cytometry troubleshooting has become an increasingly perfect tool for cell sorting and analysis. To know about ideal flow cytometry troubleshooting online you can search the websites of flow cytometry explanation online.
At the moment, flow cytometry has been extensively utilized in clinical medicine and essential medical research areas such as immunology, oncology, cell biology, hematology, cytogenetics, biochemistry, etc. In June, our section introduced a FACSCalibur flow cytometer, which was put into a clinical program, providing a great means for clinical investigation and scientific study.
Flow cytometry (flowcytometryFCM), also referred to as flow cytometry.
The principle is that the cells to be analyzed are stained with specific fluorescent dyes and put in a sample tube, and then enter the flow chamber full of sheath fluid under the pressure of this gas.
Under the restriction of the sheath fluid, the cells are arranged in one row and ejected from the nozzle of the flow chamber to form a column of cells. The latter intersects the incident laser beam perpendicularly. The cells in the column of liquid are excited by the laser to create fluorescence.
A string of optical methods (lenses, filters, diaphragms, and sensors ) from the instrument collects signals like fluorescence, brightness scattering, light absorption, or mobile electrical impedance.
The computer practice collects, stores, displays, and analyzes the estimated signals, and makes analytical analysis of various indexes.